发现 生活与效率 相关 Skills，比较来源仓库和用途，查看适合你工作流的技能说明。
Performs fine-grained re-clustering on specific subsets of cells (e.g., individual clusters, cell types, or custom subsets). Unlike `Seurat::FindSubCluster` which only finds subclusters within a single cluster,...
Identifies and visualizes the top expressing genes per cluster in T/B cells, followed by pathway enrichment analysis. Provides quick cluster characterization by highlighting the most highly expressed genes and...
TESSA (TCR and Expression Joint Clustering) is a Bayesian model that integrates T-cell receptor (TCR) sequence profiling with transcriptomes of T cells. It maps the functional landscape of the TCR repertoire by...
Load, prepare, and apply quality control (QC) to single-cell RNA-seq data using Seurat. Performs data loading, QC filtering, normalization, and multi-sample integration. This is a core preprocessing process tha...
Load single-cell TCR-seq or scBCR-seq data from various formats into a scRepertoire-compatible object. This process reads VDJ (variable, diversity, joining) receptor contig data from multiple single-cell sequen...
Separates T and non-T cells or B and non-B cells from a mixed cell population. Uses either clonotype percentage from VDJ data, indicator gene expression (CD3 markers for T cells, CD19/CD20 for B cells), custom...
Performs unsupervised clustering on single-cell RNA-seq data using Seurat. This process finds nearest neighbors, computes UMAP for visualization, and applies Louvain/Leiden algorithms to identify cell clusters....
Imputes missing/dropout values in scRNA-seq expression data to improve metabolic pathway analysis. This process handles sparsity common in single-cell RNA sequencing data by filling in zero values using advance...
Annotates cell clusters with biological cell type labels using multiple methods: direct assignment, ScType, scCATCH, hitype, or CellTypist. This process is essential for interpreting clustering results by assig...
Comprehensive metabolic landscape analysis pipeline for scRNA-seq data. This is an all-in-one process group performing complete metabolic pathway analysis including expression imputation, feature selection, pat...
Performs coarse clustering on ALL cells (including T cells, B cells, and non-T/B cells) before cell type selection. This process identifies broad cell populations to enable subsequent T/B cell selection via `TO...
Finds marker genes for clusters of ALL cells before T/B cell selection. This process identifies differentially expressed genes across unsupervised clusters to help identify broad cell types (T cells, B cells, M...
Finds differentially expressed genes (markers) for clusters of T/B cells using Seurat's FindMarkers function. Performs statistical testing between clusters, identifies cluster-defining genes, and automatically...
Performs pseudo-bulk differential gene expression analysis using DESeq2 or edgeR. Aggregates single-cell counts to sample-level pseudo-bulk data, then identifies differentially expressed genes between condition...
Analyzes metabolic pathway heterogeneity within cell populations by calculating normalized enrichment scores (NES) for each pathway across different groups. Quantifies metabolic diversity and identifies pathway...
Infer ligand-receptor interactions and cell-cell communication networks from single-cell RNA-seq data using the LIANA+ framework. Identifies potential signaling events between cell types based on gene expressio...
基于 PPT 模板生成新内容。PDF 自动转图片 → 分析模板风格 → 拆分文章内容 → 生成提示词 → AI 生图 → 打包 PPTX。
Rust 技能索引。提供所有技能的快速导航和查询入口。触发词：skill, index, 技能, 索引, 目录
Comprehensive vitest testing patterns covering test structure, AAA pattern, parameterized tests, assertions, mocking, test doubles, error handling, async testing, and performance optimization. Use when writing,...
性能优化专家。处理 benchmark, profiling, allocation, SIMD, cache, 优化, 基准测试等问题。触发词：performance, optimization, benchmark, profiling, allocation, SIMD, cache, make it faster, 性能优化, 基准测试, 内存...
嵌入式 C 代码风格助手。基于 51 单片机例程学习的代码规范, 用于创建项目和优化代码。
高级生命周期专家。处理 HRTB, GAT, 'static 约束, dyn trait object, 泛型边界冲突等问题。触发词：lifetime, HRTB, GAT, 'static, dyn, trait object, one type is more general, 生命周期, 类型约束
并发与异步专家。处理 Send, Sync, thread, async, await, tokio, channel, Mutex, deadlock, race condition, 并发, 异步, 死锁等问题。触发词：thread, spawn, channel, mpsc, Mutex, RwLock, Atomic, async, await, Future,...
高级异步模式专家。处理 Stream 实现, 零拷贝, tokio::spawn 生命周期, 插件系统调度, tonic 流式响应等问题。触发词：async, Stream, tokio::spawn, 零拷贝, 插件系统, tonic, 流式, BorrowedMessage, 异步调度
默认按已收录数据中的 GitHub 仓库 Stars 排序。Stars 属于来源仓库，同一仓库的多个技能可能共享相同数值；它反映仓库关注度，不代表单个技能的安装量或质量评分。